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Molecular Biology Products-PCR Taq

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  • Where can I find help troubleshooting my PCR?
  • Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
  • When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
  • The product sequence doesn't completely match the expected sequence. How can this result be improved?
  • Why is there no product when visualized on an agarose gel?
  • What type of DNA end results from a primer extension reaction or a PCR using Taq DNA Polymerase?
  • What is the maximum product length that can be made by GenScript's Taq DNA Polymerase?
  • What is the proper concentration for a routine PCR reaction?
  • How should I set up an amplification reaction using Taq DNA Polymerase?
  • Which buffer should I use if I want to control the level of magnesium (Mg2+) in the reaction? Does the presence of Mg2+ inhibit PCR?
  • Can Taq DNA Polymerase be used in other buffers?
  • Can Taq DNA Polymerase or Green Taq be used to amplify GC-rich amplicons?
  • How is Taq different from Green Taq?
  • What is the recommended enzyme amount when using Taq DNA Polymerase or Green Taq DNA Polymerase?
  • What characteristics does GenScript Taq DNA polymerase have?
  • What are the functions of Taq DNA polymerase?
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