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Using ssDNA as CRISPR HDR Precise Gene Knock-In Template

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  • How to choose from various HDR templates when performing CRISPR mediated gene KI? Should I choose dsDNA, plasmid DNA, or ssDNA?
  • What are the tips for designing an effective ssDNA template?
  • What is the recommended homology arm length on each side of the template DNA when designing ssDNA?
  • Any tips for improving CRISPR KI efficiency?
  • Any tips for generation of conditional alleles in mouse?
  • What if low HDR efficiency is detected in transfected cells?
  • What is the maximum ssDNA length GenScript produces?
  • What is the maximum quantity GenScript provides?
  • What are the quality control tests you provide for the ssDNA templates?
  • Why do you do double-sequencing, including the final ssDNA product?
  • How do you purify the final ssDNA product?
  • Do I need to worry about dsDNA contamination in the ssDNA product?
  • Why is there more than one band in the gel image on my COA report?
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