Using ssDNA as CRISPR HDR Precise Gene Knock-In Template
- How to choose from various HDR templates when performing CRISPR mediated gene KI? Should I choose dsDNA, plasmid DNA, or ssDNA?
- What are the tips for designing an effective ssDNA template?
- What is the recommended homology arm length on each side of the template DNA when designing ssDNA?
- Any tips for improving CRISPR KI efficiency?
- Any tips for generation of conditional alleles in mouse?
- What if low HDR efficiency is detected in transfected cells?
- What is the maximum ssDNA length GenScript produces?
- What is the maximum quantity GenScript provides?
- What are the quality control tests you provide for the ssDNA templates?
- Why do you do double-sequencing, including the final ssDNA product?
- How do you purify the final ssDNA product?
- Do I need to worry about dsDNA contamination in the ssDNA product?
- Why is there more than one band in the gel image on my COA report?