Using CRISPR RNP Format for Genome Editing
- How many sgRNA or crRNA sequences are needed for targeted knock-out?
- What are the advantages of using synthetic sgRNA for gene editing compared to the traditional plasmids or virus based gRNA delivery methods?
- What is the different between using synthetic sgRNA vs using crRNA: tracrRNA duplex?
- What is the advantage of your sgRNAs compared to sgRNA synthesized from in vitro transcription?
- Why should I choose modified sgRNA versus unmodified versions?