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Using CRISPR RNP Format for Genome Editing

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  • How many sgRNA or crRNA sequences are needed for targeted knock-out?
  • What are the advantages of using synthetic sgRNA for gene editing compared to the traditional plasmids or virus based gRNA delivery methods?
  • What is the different between using synthetic sgRNA vs using crRNA: tracrRNA duplex?
  • What is the advantage of your sgRNAs compared to sgRNA synthesized from in vitro transcription?
  • Why should I choose modified sgRNA versus unmodified versions?
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