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Using CRISPR Plasmids for Genome Editing

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  • What are the factors that can affect CRISPR targeting efficiency and specificity?
  • What expression vectors should I use?
  • What are your CRISPR plasmid delivery specifications?
  • How many gRNA sequences are needed for targeted knock-out?
  • How should gRNA sequences be designed?
  • Do I need an all-in-one or dual vector system?
  • When are lentiviral or adeno-associated viral (AAV) vectors necessary?
  • When should I use SpCas9 nickase vectors?
  • How are gRNA sequences designed for the transcription activation (SAM) system?
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