Sequencing
- What information needs to be filled in for sequencing?
- What files are included in the data reports provided by the sequencing service and what software does each file need to open?
- What are the requirements for DNA samples submitted for sequencing?
- What are the requirements for different types of samples?
- What are the reasons for the overlapping peaks in the results?
- What are the reasons for the failure of sequencing?
- What are the reasons for the emergence of double-peaks?
- We have verified the insertions in the samples before submitting orders. Why the sequencing result indicates an empty vector?
- There is a heterozygous site in my DNA sample, why I could not find in the sequencing report?
- There is a heterozygous locus on my sample. Why can't I see the signal of heterozygosity in my report?
- The result of sequencing is not comparable with my theoretical sequence. What is the reason?
- PCR product electrophoresis detection band is single. Why does the sequencing result say that template is miscellaneous?
- In what concentration do you require DNA samples to be submitted?
- In sequencing results, the sequence after the primer was not found. Why?
- If client does not know either the restriction enzyme sites flanking the genes or the vector map, but could provide the gene sequence and primer sequences to be used for sequencing confirmation, can you take the project?
- I want to repeat the test. Are the samples / primers still available?
- How should I quantify my DNA to meet the standard of your concentration for sequencing?
- How many primers do we recommend for the clients to order for normal PCR experiments?
- How do the walking primers return?
- How are DNA fragments over 6Kb sequenced?
- Every one of my purification products has been electrophoresis detection, and the strip running out can not be clearer. Why the sequencing test will not have a stripe?
- Does Genscript provide services for DNA sequencing, result analysis, and interpretation between two or more samples?
- Are there any differences between the hand-mixed and machine-mixed methods for random primers?
- Are there any differences between sequencing PCR product directly and the clone product indirectly?
- Although the sample has been verified before, why the sequencing result would show an empty plasmid with inserted fragments?
- Although no problem was found in each MS detection for primers of Genscript, why missing base-pair can still be found after PCR and sequencing?