Sequencing
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
- Why is the sequence of primers in the sequencing results different from the expected sequence?
- Why is my gene sequence different from the standard sequence?
- Why I got a sequencing result that was not I want?
- Why does the sequencing primer need to be site-specific on the DNA template?
- Why does my sequencing fail?
- Why did I succeed in cultivating bacteria and you failed?
- Why do I detect samples that are fairly high? Why is the feedback low?
- Why can't we find the primer in the sequencing results?
- Why can’t I find the sequence immediately after the primer in the result?
- Which format should I submit the plasmid samples in?
- Which format is better when providing DNA sequencing samples?
- Which experiments can the extracted plasmid be used specifically?
- What’s the optimal storage condition for primers and valid time for stability?
- What types of samples can be sent?
- What type of impurities remained after primers are purified? How can we minimize the residue if client is really strict with the residue?
- What software do I need to open your sequencing result?
- What may result in no final productes observed after PCR reaction?
- What is your sequencing method?
- What is your sequencing kit?
- What is the reason that bacterial cultures did not grow any colonies?
- What is the accuracy of sequencing result using your automatic fluorescence sequencing machine?
- What is the accuracy of full automatic fluorescence sequencing?
- What is primer walking sequencing? And what about the charge?
- What is base deficiency?