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Sequencing

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  • Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
  • Why there is a difference between my sequencing data and the one from the references?
  • Why the short PCR product is not suitable for direct sequencing?
  • Why target gene products are seen in control sample with no template?
  • Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
  • Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
  • Why is the sequence of primers in the sequencing results different from the expected sequence?
  • Why I got a sequencing result that was not I want?
  • Why does the sequencing primer need to be site-specific on the DNA template?
  • Why does my sequencing fail?
  • Why did I succeed in cultivating bacteria and you failed?
  • Why do I detect samples that are fairly high? Why is the feedback low?
  • Why can't we find the primer in the sequencing results?
  • Why can’t I find the sequence immediately after the primer in the result?
  • Which format should I submit the plasmid samples in?
  • Which format is better when providing DNA sequencing samples?
  • What’s the optimal storage condition for primers and valid time for stability?
  • What types of samples can be sent?
  • What type of impurities remained after primers are purified? How can we minimize the residue if client is really strict with the residue?
  • What software do I need to open your sequencing result?
  • What may result in no final productes observed after PCR reaction?
  • What is your sequencing method?
  • What is your sequencing kit?
  • What is the reason that bacterial cultures did not grow any colonies?
  • What is the accuracy of sequencing result using your automatic fluorescence sequencing machine?
  • What is the accuracy of full automatic fluorescence sequencing?
  • What is primer walking sequencing? And what about the charge?
  • What is base deficiency?
  • What files are included in the data reports provided by the sequencing service and what software does each file need to open?
  • What are the requirements for DNA samples submitted for sequencing?
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