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Custom cloning

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  • Why can't I cut this plasmid with XbaI and I can successfully cut other constructs with XbaI?
  • Why customers get incorrect colonies when the CloneEZ reaction is transformed?
  • Why customers are not able to get sufficient colonies when the CloneEZ reaction is transformed?
  • Why do you add some extra bases outside of cloning sites? Do these bases interfere with any downstream applications?
  • What's the method Genscript uses for subcloning?
  • What is your definition of “subcloning” , “PCR cloning” and “customized cloning”?
  • What is your cloning/subcloning method in generating constructs for customer?
  • What is the main difference between cloneEZ and Invitrogen Gateway cloning?
  • I need a common expression vector. Do you have any in stock?
  • What E.coli competent do you usually use for cloning?
  • What is the antibiotic resistance of the vector pUC57?
  • There is a restriction site in my gene, but I want to clone the gene into a vector using this site. Can you clone it for me?
  • On the order form, it requires cloning sites. Is this which cloning sites I would like the gene inserted into the vector? Also, do I have to include these sites at the both ends of my sequence when ordering?
  • If I want to do PCR cloning, what infomation do you need?
  • I have a complicated cloning project, do I need to design all of the cloning steps?
  • Does Genscript provide the Gateway clone technology service for me?
  • Can you tell me the price about the PCR cloning?
  • Can we change the target vector after mutagenesis?
  • Can I submit PCR product as starting material for subcloning?
  • Can you clone a gene into your vector pUC57 simple between the NdeI-HindIII region ?
  • Can Genscript clone the gene directly to my own vector but not the pUC57?
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