Protein
- What if the protein can't be expressed and purified in E. coli system?
- What is the concentration of protein that you usually get from E.coli expression system?
- For antibody generation, are there special requirements for the production of antigen protein?
- I want to express a heterodimer constitutes with two subunits. How would you suggest, in one construct or in two constructs?
- What is "crystallographic grade" protein?
- I may want to order large scale protein production later, but not now. How long will GenScript keep the expression vector for me?
- What is the best temperature for shipping and long term storage of Baculovirus (BV)? Is there any difference in the storage buffer for delivery and long term storage?
- What QC data do you provide for Baculovirus Preparation Service (SC1261)?
- Do you regularly check endotoxin level for custom proteins?
- Why shall I choose GenScript to produce custom protein expression?
- Do you ship the produced protein in lyophilized or liquid form?
- What is the cost and time for scale up?
- Why GenScript usually do 1L pilot to determine the price for scale up?
- Can we remove the tag after purification? If so, how much will it cost and how long will it take?
- What tags does GenScript use most often in order to increase solubility? What tags do GenScript use most often for purification purpose?
- Why does GenScript always add some tags at the target protein?
- Can you use our own expression vectors? If so, what information should we provide?
- What QC data do you provide for E. coli expression?
- What's the timeline for insect expression and purification?
- Could you provide me with a quote regarding to certain quantity of protein (eg. 1g with 95% purity)?
- Does codon optimization really matter?
- How can I choose an expression system for my protein?
- Where is the HIS tag going to be added for bacterial system?
- I don't have the genes ready for these proteins. Can you synthesize them for me? Is there any detailed information about the gene you need?
- What affect the cost of the gene synthesis and expression?
- Is there only one clone picked for E. coli expression test?
- Can I provide the vector containing the gene and let you express it for me?
- Did you have any difficulty in your expression and purification experiences?
- Will you purify the protein from the soluble fraction or insoluble fraction of the cell lysate? Can you refold the protein?
- I will provide you with the plasmid for expression. What quantities of the plasmid will you require?