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Protein

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  • What is the concentration of protein that you usually get from E.coli expression system?
  • For antibody generation, are there special requirements for the production of antigen protein?
  • I want to express a heterodimer constitutes with two subunits. How would you suggest, in one construct or in two constructs?
  • What is "crystallographic grade" protein?
  • I may want to order large scale protein production later, but not now. How long will GenScript keep the expression vector for me?
  • What is the best temperature for shipping and long term storage of Baculovirus (BV)? Is there any difference in the storage buffer for delivery and long term storage?
  • Do you regularly check endotoxin level for custom proteins?
  • Why shall I choose GenScript to produce custom protein expression?
  • Do you ship the produced protein in lyophilized or liquid form?
  • What is the cost and time for scale up?
  • Why GenScript usually do 1L pilot to determine the price for scale up?
  • Can we remove the tag after purification? If so, how much will it cost and how long will it take?
  • What tags does GenScript use most often in order to increase solubility? What tags do GenScript use most often for purification purpose?
  • Why does GenScript always add some tags at the target protein?
  • What QC data do you provide for E. coli expression?
  • What's the timeline for insect expression and purification?
  • Could you provide me with a quote regarding to certain quantity of protein (eg. 1g with 95% purity)?
  • Does codon optimization really matter?
  • How can I choose an expression system for my protein?
  • Where is the HIS tag going to be added for bacterial system?
  • I don't have the genes ready for these proteins. Can you synthesize them for me? Is there any detailed information about the gene you need?
  • What affect the cost of the gene synthesis and expression?
  • Is there only one clone picked for E. coli expression test?
  • Can I provide the vector containing the gene and let you express it for me?
  • Did you have any difficulty in your expression and purification experiences?
  • Will you purify the protein from the soluble fraction or insoluble fraction of the cell lysate? Can you refold the protein?
  • I will provide you with the plasmid for expression. What quantities of the plasmid will you require?
  • What is the difference between Ni-affinity column and Co-affinity column?
  • What HA sequence do you usually use?
  • If expression level is too low to get purified, how would you charge me?
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