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Antibody

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  • I want to develop two phospho-specific pAbs, each one of them against one phospho-site. However, these two phosphorylated sites locate very close. Is it possible to get two specific pAbs that only recognize one phospho-site with no reaction to the other?
  • Will Genscript ship some samples for test before final delivery?
  • Why there is no affinity purification service for mouse pAb package?
  • Why there is an extra cys in the antigen sequence you designed for me? Is it necessary to conjugate the peptide to a carrier protein and why?
  • Why there is no immune-affinity purification option in mAb purification?
  • Why is the antibody yield so much higher for the protein A-purified package verses the other purification methods?
  • Why do you need to do KLH conjugation for my antigen? What carrier protein should I choose for conjugation?
  • Why do I order test bleed?
  • Why am I seeing multiple or unexpected bands on a Western with my affinity purified antibody?
  • Which end of the peptide “C” should be added to for KLH conjugation?
  • Where can clients get the ISO9001 document and OLAW?
  • What starting material should I offer for Anti-idiotype Monoclonal Antibody service?
  • What purity of the protein antigen is acceptable to you? What buffer should I use?
  • What purification methods do you provide for pAbs?
  • What kind of starting material do you need for antibody production from customer provided hybridoma cell lines?
  • What kind of antigen you can accept for hybridoma generation?
  • What is the typical turnaround time for pAbs? Do you provide expedited services?
  • What is the timeline for hybridoma development?
  • What is the difference between affinity purification and protein A/G purification? Which one would you recommend?
  • What is the concentration of the Ab mentioned on the COA I received for my custom Ab order? Am I supposed to use these figures for reconstitution?
  • What is the antibody yield from roller bottle supernatant?
  • What is the antibody yield from ascites fluid?
  • What is phosphor-specific antibody?
  • What is an adjuvant and what adjuvants do you use?
  • What are the mouse species and fusion partner you used to generate the hybridomas?
  • What are the features of rabbit monoclonal antibody (mAb) when compare to mouse mAb?
  • What AA can be methylated? At what group?
  • Is your mouse SPF mouse?
  • Is it possible to do ascites production for rat mAb production cell line?
  • In order to get the antibody specific to target protein with {Lys(me3)}, can you use peptide with {Lys(me3)} for immunization and use {Lys(me2)} & {Lys(me)} & non-modified peptide for cross adsorption (pAb) or counter screening (mAb)?
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