Catalog Product
Different kinds of Catalog products are available to better assist your research and accelerate the R & D process
Catalog Protein
- Do most proteins show cross-species activity?
- Can I use recombinant proteins from different companies for my ELISA?
- I want to try to do an experiment with your protein, but the bioassay you use for determining activity is not the same as my application. Will my application work with your protein?
- Does the specific activity of a recombinant protein vary between lots?
- How does the activity of your recombinant proteins compare to competitors’? Do you test the bioactivity of your recombinant proteins with in vivo assays?
- What is the specific activity of your recombinant proteins? What is meant by a “unit” of protein activity?
Molecular Biology Products-PCR Taq
- Where can I find help troubleshooting my PCR?
- Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
- When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
- The product sequence doesn't completely match the expected sequence. How can this result be improved?
- Why is there no product when visualized on an agarose gel?
- What type of DNA end results from a primer extension reaction or a PCR using Taq DNA Polymerase?
Molecular Biology Products-PCR Cloning Kit
- What is the stability of the enzyme included in GenBuilder™ PCR Cloning Kit?
- Will the GenBuilder™ reaction work more efficiently if I use primers that contain a longer than 15 base region of homology?
- What factors need to be considered when using GenBuilder™ PCR Cloning Kit with customers' vector?
- Can I use GenBuilder™ PCR Cloning Kit with my own vectors?
- Can multiple fragments be cloned into a single vector using this kit?
- What should the purity of my primer be to be compatible with the GenBuilder™ cloning method?
Molecular Biology Products-Plasmid Purification
- Can I extract and purify DNA from low melting point (LMP) Agarose gels?
- Can I extract and purify DNA from gels using TAE running buffer?
- What additional consumables does the user need?
- Can I use TE buffer or water to elute DNA from the column?
- Is it necessary to repeat the wash procedure?
- Will QuickClean II PCR Purification Kit remove fluorescent dyes from real-time PCR reactions?
Molecular Biology Products-GenBuilder™
Protein Isolation and Electrophoresis-Precast Gels
- Will overnight destaining affect the resolution of the band?
- Why do some parts of the gel become sticky following protein transfer?
- What's the buffer I should use to transfer the gel?
- What running buffer should I use to run the gel?
- Which sample loading buffer should I choose?
- Is this gel stable at room temperature?
Catalog Antibody
- How is PIN1 fused to its partner? (A01090 PIN1 Antibody, pAb, Rabbit)
- Is there any cross-reactivity between the fusion partner and PIN1? (A01090 PIN1 Antibody, pAb, Rabbit)
- What is the fusion partner? (A01090 PIN1 Antibody, pAb, Rabbit)
- The MW of the target protein is about 18 kDa, so why is the band in the WB test picture almost 60 kDa. (A01090 PIN1 Antibody, pAb, Rabbit)
- In which species does antibody A01622 (GAPDH Antibody, mAb, Mouse) recognize GAPDH?
- Does Catalog No. A00160 (Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb) cross react with rabbit?
Endotoxin Kits
His Tag Protein Purification
- What concentration of PMSF is needed for cell lysis and protein purification?
- The manual mentions that the Lysis Equilibration Buffer, Wash Buffer and Elution buffer contain 50 mM NaH2PO4 and 300 mM NaCl. Is it possible to use 50mM PBS instead of them?
- Is it necessary to regenerate the column each time?
Cell Line Product
- Do you know if the cell line will remain stable in absence of hygromycin?
- Do you provide the sequence of the inserted gene that was used for the generation of the stable cell line?
- Have you ever evaluate reducing the level of FBS (eg 10 → 5%)
- Can we omit the wash step before the addition of trypsin-EDTA/Accutase? We usually do not perform this step.
- Have you evaluated Accutase in replacement for trypsin-EDTA?
- What do you mean by stability 15 passages? Have you observed any decrease in expression for functionality after 15 passages?