Molecular Biology
100% sequence-verified genes delivered in as short as 4 days.
General
- What information do you need to prepare a quote?
- Why is my gene sequence different from the standard sequence?
- If client does not know the restriction enzyme sites flanking the genes, nor the vector map, but could provide the gene sequence, and primer sequences to be used for sequencing confirmation, can we take the project?
- With what vectors do we ship bacstab & induction solution to the client?
- Why did my PCR reaction stop working?
- What's the plasmid shipping temperature and storage temperature ?
Codon optimization
- Why do I need codon optimization?
- Why CAI (Codon Adaptation Index) of my gene after optimization is smaller than the CAI before optimization?
- Which stop codon is more preferable in E coli expression system? How about mammalian system?
- What is common kozak sequence for mammalian expression?
- What information do you need for optimization?
- What else does gene optimization do besides codon optimization? (Why CAI after optimization is smaller than the CAI before optimization?)
Gene synthesis
- Why do I see extra bands in my PCR product?
- Which vector do you use when synthesizing the gene?
- Which software can I use to open the sequence files of the synthesized gene?
- What QC data do I get for gene synthesis service?
- What options does GenScript offer for gene deliverables?
- What kind of bacterial strain is used in gene synthesis service and how should it be handled?
Mutagenesis
- Which protocol do you use for mutagenesis, is it quick change?
- What product will you deliver to me when the mutagenesis project completed?
- What material should we provide for the mutagenesis?
- What is the sequence size limit for one point mutation?
- What is the price for inserting a fragment and deleting a fragment?
- I have never sequenced my template, can you still perform mutagenesis for me?
Custom cloning
- Why can't I cut this plasmid with XbaI and I can successfully cut other constructs with XbaI?
- What's the method Genscript uses for subcloning?
- What is your definition of “subcloning” , “PCR cloning” and “customized cloning”?
- What is your cloning/subcloning method in generating constructs for customer?
- What is the main difference between cloneEZ and Invitrogen Gateway cloning?
- I need a common expression vector. Do you have any in stock?
Plasmid preparation
- What QC is done after plasmid prep?
- What information should I provide for the plasmid preparation?
- What bacteria strain do you use to grow DNA?
- How much could it cost to the plasmid preparation?
- Does your lab do DNA extraction, isolation, cleaning, and purification?
- Do you ship the plasmid prep in lyophilized or in liquid form? If liquid, what buffer is used and what is the concentration?
Mutation library
- What is the diversity of randomized and degenerate library?
- How do you confirm the library size? The variety of the library?
- How to calculate the diversity of a site-directed or permutation scanning library?
- How do you make site-directed or permutation scanning library?
- How do you make randomized and degenerate libraries?
Sequencing
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
Oligo
- Why should oligonucleotides be purified?
- Why does MALDI analysis of my oligos containing one or more Fluorescein-dTs give an incorrect mass even though they give only a single, fluorescent band on a PAGE gel?
- What shall I do with the RNA synthesis product from your company?
- What exactly is an OD?
- Sometimes I notice that the dried oligos have a brown color after drying. Is this the natural color of DNA or is the brown color a sign of contamination?
- I used oligos for cloning. When I sequenced a clone and found a mutation within the oligo sequence. Can mutations happen in synthetic oligos?