Molecular Biology
100% sequence-verified genes delivered in as short as 4 days.
Others
- How do you determine/confirm the library size or the variety of the library?
- With what vectors do we ship bacstab & induction solution to the client?
- Why did my PCR reaction stop working?
- What's the plasmid shipping temparature and storage temperature ?
- What is order confirmation, why do I need to confirm?
- The template is from our previous order from GenScript, do you still have the plasmid or I need to provide it again?
Sequencing
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
Mutation library
Plasmid preparation
- What QC is done after plasmid prep?
- What information should I provide for the plasmid preparation?
- What bacteria strain do you use to grow DNA?
- How much could it cost to the plasmid preparation?
- Does your lab do DNA extraction, isolation, cleaning, and purification?
- Do you ship the plasmid prep in lyophilized or in liquid form? If liquid, what buffer is used and what is the concentration?
Mutagenesis
- Which protocol do you use for mutagenesis, is it quick change?
- What product will you deliver to me when the mutagenesis project completed?
- What material should we provide for the mutagenesis?
- What is the sequence size limit for one point mutation?
- What is the price for inserting a fragment and deleting a fragment?
- I have never sequenced my template, can you still perform mutagenesis for me?
Custom cloning
- Why can't I cut this plasmid with XbaI and I can successfully cut other constructs with XbaI?
- Why customers get incorrect colonies when the CloneEZ reaction is transformed?
- Why customers are not able to get sufficient colonies when the CloneEZ reaction is transformed?
- Why do you add some extra bases outside of cloning sites? Do these bases interfere with any downstream applications?
- What's the method Genscript uses for subcloning?
- What is your definition of “subcloning” , “PCR cloning” and “customized cloning”?
Oligo
- Why should oligonucleotides be purified?
- Why does MALDI analysis of my oligos containing one or more Fluorescein-dTs give an incorrect mass even though they give only a single, fluorescent band on a PAGE gel?
- What shall I do with the RNA synthesis product from your company?
- What exactly is an OD?
- Sometimes I notice that the dried oligos have a brown color after drying. Is this the natural color of DNA or is the brown color a sign of contamination?
- I used oligos for cloning. When I sequenced a clone and found a mutation within the oligo sequence. Can mutations happen in synthetic oligos?
GenParts
Gene synthesis
- Why do I see extra bands in my PCR product?
- Which vector do you use when synthesizing the gene?
- Which software can I use to open the sequence files of the synthesized gene?
- What QC data do I get for gene synthesis service?
- What options does GenScript offer for gene deliverables?
- What kind of bacterial strain is used in gene synthesis service and how should it be handled?
Codon optimization
- Why do I need codon optimization?
- Why CAI (Codon Adaptation Index) of my gene after optimization is smaller than the CAI before optimization?
- Which stop codon is more preferable in E coli expression system? How about mammalian system?
- What is common kozak sequence for mammalian expression?
- What information do you need for optimization?
- What else does gene optimization do besides codon optimization? (Why CAI after optimization is smaller than the CAI before optimization?)