Here are the guidelines for a 50 μl PCR reaction:
- 0.5 μl Taq DNA Polymerase
- 1 μl 10 mM dNTP
- 1 μl each primer
- 2 μl genomic template (up to 100 ng/μl)
- 5 μl 10x Taq Buffer (Standard Taq containing Mg2+)
- 39.5 μl sterile or filtered H2O
- Denature at 94°C
- Extend 1 minute/kb