Why is my plasmid DNA yield low? March 10, 2021 08:39 Check if the bacteria was cultured properly. Check if bacteria cells were resuspended completely. Incubate the Elution Buffer at 30-60°C，this should help increase yields. Related articles Why should I choose GenWandTM dsDNA over PCR dsDNA? What is the difference between Net Peptide Weight and Gross Peptide Weight? What is the diversity of randomized and degenerate library? What is Endotoxin? Can I use recombinant proteins from different companies for my ELISA?