GenScript has four types of fluorophore conjugated His-tag antibodies for FACS analysis, including A01620 (FITC), A01800 (iFluor488), A01801 (iFluor555) and A01802 (iFluor 647). The iFluor series are recommended for FACS analysis because they have a higher sensitivity than FITC conjugated antibody. The following is the wavelength range of GenScript's iFluor antibodies:
|
Cat. No.
|
Conjugate
|
Ex (nm)
|
Em (nm)
|
|---|---|---|---|
|
A01800
|
iFluor 488
|
491
|
514
|
|
A01801
|
iFluor 555
|
559
|
569
|
|
A01802
|
iFluor 647
|
654
|
674
|
According to our FACS analysis results, the sensitivity of A01802 (THE™ His Tag Antibody [iFluor 647], mAb, Mouse) is slightly higher than the A01800 (THE™ His Tag Antibody [iFluor 488], mAb, Mouse). If the His Tag number is low, A01802 is recommended.