Yes. Other detergents and high levels of chaotropes (urea and guanidine) can reduce the affinity of Polymixin B for LPS. Proteins such as BSA can bind tightly to endotoxin, reducing its ability to interact with and bind to the endotoxin Removal Resin. This reduction in binding capacity can sometimes be overcome by increasing the volume of immobilized polymixin B. However, some proteins bind tightly to endotoxin without inhibiting its binding to ligands. In this case, the protein will remain bound to the resin with the endotoxin, possibly causing a loss of the protein of interest.
