What concentration of PMSF is needed for cell lysis and protein purification? March 10, 2021 05:37 We recommend a final concentration of PMSF at 1 mM. Related articles Does any substance interfere with the binding of endotoxin to endotoxin Removal Resin? The manual mentions that the Lysis Equilibration Buffer, Wash Buffer and Elution buffer contain 50 mM NaH2PO4 and 300 mM NaCl. Is it possible to use 50mM PBS instead of them? What is the maximum acceptable endotoxin level? Do most proteins show cross-species activity? Is there a COA in the shipping package? What tests are included in COA?