What concentration of PMSF is needed for cell lysis and protein purification? March 10, 2021 05:37 We recommend a final concentration of PMSF at 1 mM. Related articles The manual mentions that the Lysis Equilibration Buffer, Wash Buffer and Elution buffer contain 50 mM NaH2PO4 and 300 mM NaCl. Is it possible to use 50mM PBS instead of them? Does any substance interfere with the binding of endotoxin to endotoxin Removal Resin? Is it necessary to regenerate the column each time? I am new to CRISPR technology and need technical support, how can GenScript help me with that? Here is the sequence of the peptide 'QRPRLSHKGPMP', can you make it?