The manual mentions that the Lysis Equilibration Buffer, Wash Buffer and Elution buffer contain 50 mM NaH2PO4 and 300 mM NaCl. Is it possible to use 50mM PBS instead of them? March 10, 2021 05:36 The concentration of PBS is lower. It will result in some non-specific binding. Related articles Is it necessary to regenerate the column each time? What concentration of PMSF is needed for cell lysis and protein purification? Does any substance interfere with the binding of endotoxin to endotoxin Removal Resin? Do you know if the cell line will remain stable in absence of hygromycin? Do you provide the sequence of the inserted gene that was used for the generation of the stable cell line?