What concentration of PMSF is needed for cell lysis and protein purification?
The manual mentions that the Lysis Equilibration Buffer, Wash Buffer and Elution buffer contain 50 mM NaH2PO4 and 300 mM NaCl. Is it possible to use 50mM PBS instead of them?
Is it necessary to regenerate the column each time?

Is it necessary to regenerate the column each time?

March 10, 2021 05:34

No. You can regenerate the column every 3-5 times.