Please check the certificate of analysis included with your shipment for the exact instructions for reconstitution because not all products are reconstituted with the same conditions. In general, we recommend using sterile water for reconstitution. Add the recommended volume of sterile water to the vial, and gently shake it to solubilize the protein completely. Do not vortex.
Typically, 100 µL is an acceptable reconstitution volume for our 10 µg and 50 µg vials of recombinant protein. Our 10 µg and 50 µg vials have a maximum volume of 500 µL. Typically, 1 mL is an acceptable reconstitution volume for our 100 µg vials of recombinant protein. Our 100 µg vials have a maximum volume of 2 mL.
The concentration of recombinant protein is dependent on the reconstitution volume. For example, if reconstituting 10 µg of recombinant protein in 100 µL carrier protein solution, the concentration is 10 µg/100 µL or 100 µg/mL.