Where can I find help troubleshooting my PCR? – GenScript Help Center

Please send all questions to product@genscript.com.

Problem	Possible Cause(s)	Solution(s)
Little or no amplification product	GC rich template	add 5% DMSO, 1 M betaine, or both can be included in PCR reactions to improve results
	Template degradation	Analyze the template on an agarose gel to check for possible degradation
	PCR inhibitor contamination	Test the template with an established PCR system Repeat template purification to remove the remainder alcohol, Phenol or other contamination
	Cycle conditions	Change annealing temperature Increase cycle number Prolong the elongation time
	Primer problem	Both primers must have the same concentration. The final concentration must be between 0.2 µM ~ 0.6 µM Make sure that the primers are not degraded
	Low enzyme concentration	Increase the amount of polymerase in 0.5 U steps
	Low MgCl2 concentration	Increase the MgCl2 concentration in 0.25mM steps
	Inadequate annealing temperature	Increase annealing temperature
	Improper template concentration	Test serial dilution of template
	Formation of primer dimers	When the temperature of the PCR cycle reaches 94°C insert PCR reaction tube and continue the program