Where can I find help troubleshooting my PCR?
Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
The product sequence doesn't completely match the expected sequence. How can this result be improved?
Why is there no product when visualized on an agarose gel?
What type of DNA end results from a primer extension reaction or a PCR using Taq DNA Polymerase?
What is the maximum product length that can be made by GenScript's Taq DNA Polymerase?
What is the proper concentration for a routine PCR reaction?
How should I set up an amplification reaction using Taq DNA Polymerase?
Which buffer should I use if I want to control the level of magnesium (Mg2+) in the reaction? Does the presence of Mg2+ inhibit PCR?

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What is the proper concentration for a routine PCR reaction?

March 10, 2021 09:01

For up to 3 kb, we recommend 1.25 units per 50 µl reaction
For specialized applications, including 3–8 kb amplicons, 2.5 units per 50 µl reaction is recommended