Yes. GenBuilder™ PCR Cloning Kit can be used for multiple fragment recombination if there are not many repeated sequences among multiple fragments. However, we recommend recombination one fragment at a time. Several performances of recombining 2 - 3 fragments demonstrated efficiency as low as 20%-30%, and lead to a direct repeat deletion.
Articles in this section
- What is the stability of the enzyme included in GenBuilder™ PCR Cloning Kit?
- Will the GenBuilder™ reaction work more efficiently if I use primers that contain a longer than 15 base region of homology?
- What factors need to be considered when using GenBuilder™ PCR Cloning Kit with customers' vector?
- Can I use GenBuilder™ PCR Cloning Kit with my own vectors?
- Can multiple fragments be cloned into a single vector using this kit?
- What should the purity of my primer be to be compatible with the GenBuilder™ cloning method?
- What factors need to be considered when designing the primer?
- Why do I generate incorrect colonies when the GenBuilder™ reaction is transformed?
- Why don't I generate sufficient colonies when the GenBuilder™ reaction is transformed?
- Technical information is not available in the manual, why is that?