Yes. QuickClean II PCR or Gel Extraction Kit can be used to extract and purify DNA from low melting point (LMP) gels.
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Problem
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Solution
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Obtain no plasmid DNA
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If there is no plasmid DNA in the elution buffer, please check whether the ethanol had been added to wash buffer according to the volume marked on bottle label.
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Low plasmid DNA yields
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- Please check if the bacteria was cultured properly.
- Please check if the bacteria cells were resuspended completely. 3. Incubate the Elution Buffer at 30～60°C，to help increase the yields.
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Absorbance problem
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- Absorbance is the difference of the sample from the blank, please use the Elution Buffer to adjust the blank to a zero value and use it to dilute the sample.
- If the ratio of OD260/ OD230 is too low, wash the spin column for one more time.
- In the case of a low ratio of OD260-320/ OD280-320, there may be protein contamination. In this case please add Neutralization Buffer, and then centrifuge buffer with sufficient rotating speed, thus to make precipitation compact; be careful to pipette supernatant and avoid pipetting the precipitate.
- If the ratio of OD260-320/ OD280-320 is too high, add more RNase A to Resuspension Buffer to a final concentration 100 μg/ml.
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Electrophoresis problem
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- If there is genomic DNA in the result, invert the tube gently (step 3 and 4).
- If there is RNA in the result, add more RNase A to Resuspension Buffer to a final concentration 100 μg/ml.
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