What's the buffer I should use to transfer the gel? March 10, 2021 08:30 We recommend Tris Bicine transfer buffer (M00139). Related articles What running buffer should I use to run the gel? Is this gel stable at room temperature? What's the difference between ExpressPlus™ and SurePAGE™ gels? What should the purity of my primer be to be compatible with the GenBuilder™ cloning method? Can I extract and purify DNA from gels using TAE running buffer?