How is PIN1 fused to its partner? (A01090 PIN1 Antibody, pAb, Rabbit)
Is there any cross-reactivity between the fusion partner and PIN1? (A01090 PIN1 Antibody, pAb, Rabbit)
What is the fusion partner? (A01090 PIN1 Antibody, pAb, Rabbit)
The MW of the target protein is about 18 kDa, so why is the band in the WB test picture almost 60 kDa. (A01090 PIN1 Antibody, pAb, Rabbit)
In which species does antibody A01622 (GAPDH Antibody, mAb, Mouse) recognize GAPDH?
Does Catalog No. A00160 (Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb) cross react with rabbit?
Is Catalog No. A00166 (Goat Anti-Human IgG Antibody (H&L) [HRP], pAb) specific for human IgG. Does it cross-react with non-human primates or rodents?
I've purchased A01428-100 (THE™ DYKDDDDK Tag Antibody [HRP], mAb, Mouse). I was wondering if the presence of HRP will interfere with secondary antibody binding (either Goat anti-Mouse secondary or Protein G secondary).
Do you have any information on antibody stability after reconstitution? I'm concerned that an IgM antibody may have fragmented during the lyophilization process or became aggregated upon reconstitution.
Can I use A01795 (THE™ PEG Antibody, mAb, Mouse) in building an affinity column.

See more

The protein band in the western blot is different from the predicted molecular weight of the protein. What might be the possible reason?

March 10, 2021 07:55

Post-tanslational modification, such as phosphorylation, glycosylation, etc. All of these may increase the protein molecular weight.
Post-translational cleavage: for protein processing, i.e. cleavage from a precursor to a mature active form, such as occurs with a caspase precursor.
Splice variant: isoforms from alternative splicing.
Relative charged-amino acid composition (the ratio of both charged and uncharged amino acids).