The immune checkpoint cell lines are generated using a codon optimized gene sequence transfected into host cells. The antibiotic resistant cell pools are tested for cell surface expression using FACS and then cloned out using limited dilution method. The resulting single cell derived clones are again tested for high cell surface expression of the target to select the final clone from which the cell line is derived. The cell line is also checked for passed stability (15 passages), and tested for mycoplasma and viability as a part of our standard QC process.
Articles in this section
- Do you know if the cell line will remain stable in absence of hygromycin?
- Do you provide the sequence of the inserted gene that was used for the generation of the stable cell line?
- Have you ever evaluate reducing the level of FBS (eg 10 → 5%)
- Can we omit the wash step before the addition of trypsin-EDTA/Accutase? We usually do not perform this step.
- Have you evaluated Accutase in replacement for trypsin-EDTA?
- What do you mean by stability 15 passages? Have you observed any decrease in expression for functionality after 15 passages?
- Do you recommend maintaining selection pressure (Hygromycin 250ug/mL) ?
- Do your HEK293 cells contain the large antigen T of SV40 same as HEK293T cells?
- Are more tests available?
- How about the expression level of the immune checkpoint receptor in the cell lines?