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CRISPR
Using ssDNA as CRISPR HDR Precise Gene Knock-In Template

Articles in this section

How to choose from various HDR templates when performing CRISPR mediated gene KI? Should I choose dsDNA, plasmid DNA, or ssDNA?
What are the tips for designing an effective ssDNA template?
What is the recommended homology arm length on each side of the template DNA when designing ssDNA?
Any tips for improving CRISPR KI efficiency?
Any tips for generation of conditional alleles in mouse?
What if low HDR efficiency is detected in transfected cells?
What is the maximum ssDNA length GenScript produces?
What is the maximum quantity GenScript provides?
What are the quality control tests you provide for the ssDNA templates?
Why do you do double-sequencing, including the final ssDNA product?

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What if low HDR efficiency is detected in transfected cells?

March 20, 2020 13:51

You can refer to the following workflow to identify issues and solutions:

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