Articles in this section

• How to choose from various HDR templates when performing CRISPR mediated gene KI? Should I choose dsDNA, plasmid DNA, or ssDNA?
• What are the tips for designing an effective ssDNA template?
• What is the recommended homology arm length on each side of the template DNA when designing ssDNA?
• Any tips for improving CRISPR KI efficiency?
• Any tips for generation of conditional alleles in mouse?
• What if low HDR efficiency is detected in transfected cells?
• What is the maximum ssDNA length GenScript produces?
• What is the maximum quantity GenScript provides?
• What are the quality control tests you provide for the ssDNA templates?
• Why do you do double-sequencing, including the final ssDNA product?