Unlike the traditional plasmids or lentivirus delivery methods, CRISPR/Cas9 RNP are delivered as intact complexes, and do not require cellular expression, thus has many advantages.
- DNA free
- Detectable at high levels shortly after transfection
- Quickly cleared from the cell for less off-target effects
- Highly efficient even in hard-to-transfect cells
- Best for in vivo studies