We prefer shorter peptides for the following reasons. 1) We have made antibodies with longer peptides, but have found that 15-17 mers are good enough to generate antibodies. Long sequences (>20 residues) increase the chance of covering the epitope, but may also be susceptible to steric and conformational problems during peptide synthesis and/or conjugation. 2) The price for synthesis of peptide up to 15 aa is included in our complete antibody packages. If the peptide is longer than 15 aa, then there will be additional charge.
Articles in this section
- Will Genscript ship some samples for test before final delivery?
- Why there is no affinity purification service for mouse pAb package?
- Why there is an extra cys in the antigen sequence you designed for me? Is it necessary to conjugate the peptide to a carrier protein and why?
- Why there is no immune-affinity purification option in mAb purification?
- Why is the antibody yield so much higher for the protein A-purified package verses the other purification methods?
- Why do you need to do KLH conjugation for my antigen? What carrier protein should I choose for conjugation?
- Why do you choose 15mer epitopes for Ab generation?
- Why do I order test bleed?
- Why am I seeing multiple or unexpected bands on a Western with my affinity purified antibody?
- Which end of the peptide “C” should be added to for KLH conjugation?