In simple terms, the affinity purification isolates peptide specific antibodies, while protein A/G (Protein A or protein G) purificaiton purifies total immunoglobin (Ig) from the serum. So the protein A/G purified antibody contains both antigen specific antibody and endogenous Ig of the host which may not be specific to the antigen. Usually for ELISA, WB applications, protein A/G purification shall be fine. For some other applications requiring high specificity, e.g., IHC, IP, etc, affinity purification is recommended.
Articles in this section
- Will Genscript ship some samples for test before final delivery?
- Why there is no affinity purification service for mouse pAb package?
- Why there is an extra cys in the antigen sequence you designed for me? Is it necessary to conjugate the peptide to a carrier protein and why?
- Why there is no immune-affinity purification option in mAb purification?
- Why is the antibody yield so much higher for the protein A-purified package verses the other purification methods?
- Why do you need to do KLH conjugation for my antigen? What carrier protein should I choose for conjugation?
- Why do you choose 15mer epitopes for Ab generation?
- Why do I order test bleed?
- Why am I seeing multiple or unexpected bands on a Western with my affinity purified antibody?
- Which end of the peptide “C” should be added to for KLH conjugation?