Only cryo-preserve rapidly growing and dividing hybridomas (log phase hybridomas) that show more than 90% viability. Under sterile conditions, harvest the log-phase hybridoma cells in a centrifuge tube and perform a cell count using a hemacytometer and trypan blue. Centrifuge the cells at 200 g (or 1,000 rpm) for five minutes at room temperature. Carefully remove the medium and re-suspend the cell pellet in freezing medium (DMEM with 20% fetal bovine serum and 10% DMSO) to final cell density of 5-10x10^6 cells per ml and aliquot into cryovials at 1.0 ml/vial. Place the cryovials in cryo freezing containers or other suitable containers and place in freezer overnight at -80°C. The next day, transfer the cryo-vials to a liquid nitrogen tank. Alternatively, they can be shipped overnight if packed in a sufficient amount dry ice. Wear gloves and a facemask when working with liquid nitrogen tanks or frozen cryo-vials. If possible, send duplicate samples of each cell line.
Articles in this section
- How do you validate the coverage of the HCP antibody? What is the sensitivity of HCP ELISA kit?
- Can you perform HCP antigen preparation? How do you validate?
- Do you have relevant experiences in ELISA kit development?
- Does GenScript have your own animal facility?
- How many positive hits could we guarantee in our beacon single b cell platform?
- Is GenScript able to keep the remaining positive B cells to backup?
- What are the benefits of express immunization compared to conventional immunization?
- What are the benefits of GenScript's MonoRab B cell cloning platform?
- How to decide what kind of immunogen format to use for antibody development?
- What kind of immunogens can we accept in upgraded TurboMab?