1) Depend on the source (bacteria or human) of your protein.
2) For human protein, whether post-translational modification is desired: if so, mammalian and insect cell would be fit; If not, E. coli will be the choice.
1) Depend on the source (bacteria or human) of your protein.
2) For human protein, whether post-translational modification is desired: if so, mammalian and insect cell would be fit; If not, E. coli will be the choice.