We will do an expression evaluation and optimization step for all protein produciton project at pilot stage. If expression level is low, we will communicate with you if you want us to proceed to production (1 liter expression and purification, scale up production.) or not. Either way, we will only charge you the steps we performed for you. If this is a guaranteed package in E coli expression system, we will only charge you the setting up fee if we could not meet the guaranteed protein amount for you.