If FITC is directly modified at the n-terminal amino group, the Edman degradation reaction occurs in the cleavage process, as shown in the following figure. Edman degradation is the process of determining the sequence of amino acid residues from the N terminal free of a polypeptide chain. N-terminal amino acid residues were modified by phenyl isothiocyanate, and then the modified residues were cut from the polypeptide chain (the amino acids directly linked to PITC), which were identified by chromatography, and the remaining polypeptide chains (with one less residue) were recycled for the next degradation cycle.
Articles in this section
- Why it is difficult to synthesize peptide with FITC without the Ahx linker?
- Do you provide peptoid service?
- Can you cyclize my peptide by disulphide bond (cysteine bridge) while my peptide sequence contains 3 cysteines?
- Can you make branched peptides? If yes, how long would each branch be? Can you make two branches on one original peptide? If yes, how far should two branches separate from each other?
- As for the biotinylated peptide I would like to order we have to be sure that there are absolutely no toxic byproducts in those peptides. Please describe how you remove potentially toxic ingredients such as guanidine salts, DMSO etc.
- What is the size of your mini-PEG?
- Where can I find the full name of a certain peptide modification?
- Can Genscript perform KLH or BSA conjugation on peptide with no cys in sequence?
- Can you label my peptide with FITC directly at N-terminus?
- What are the advantages of PEGylation of peptides?