For bacstab or glycerol stock, we suggest the following steps:
a) Streak bacteria on LB agar plate supplemented with suitable antibiotic.
b) Isolate a single colony.
c) Inoculate it in LB containing suitable antibiotic.
d) Isolate plasmids as usual.
For bacstab or glycerol stock, we suggest the following steps:
a) Streak bacteria on LB agar plate supplemented with suitable antibiotic.
b) Isolate a single colony.
c) Inoculate it in LB containing suitable antibiotic.
d) Isolate plasmids as usual.