What is your definition of “subcloning” , “PCR cloning” and “customized cloning”?

In general, “Subcloning” refers to the process of digesting the template vector and target vector using a set of common restriction enzymes, then purifying the released insert from the template vector digestion mixture as well as the linearized target vector. And finally ligate to generate the desired construct. “PCR cloning” is a type of “customized cloning”. Any cloning that does not fall into “subcloning” category belongs to “Customized cloning”. The term “PCR cloning” is no longer used. From the perspective of GenScript’s pricing structure, only the subcloning performed on templates that were previously synthesized by GenScript is considered as “subcloning” and is priced at $149/subcloning, for genes less than 3kb in length. All cloning work performed on customer provided template is considered “customized cloning”.