What is your definition of “subcloning” , “PCR cloning” and “customized cloning”?

In general, “subcloning” refer to a process, in which the template vector and target vector are digested using a set of common restriction enzymes, followed by the purification of the released insert from the template vector digestion mixture as well as the linearized target vector, and completed by the final ligation to generate the desired construct. “PCR cloning” is a type of “customized cloning”. Any cloning that does not fall into “subcloning” category belongs to “customized cloning”. The term “PCR cloning” is no longer used. From the perspective of GenScript’s pricing structure, only the subcloning performed on templates that were previously synthesized by GenScript is considered as “subcloning”. All cloning work carried out on customer provided template is considered “customized cloning”.