In general, “subcloning” refer to a process, in which the template vector and target vector are digested using a set of common restriction enzymes, followed by the purification of the released insert from the template vector digestion mixture as well as the linearized target vector, and completed by the final ligation to generate the desired construct. “PCR cloning” is a type of “customized cloning”. Any cloning that does not fall into “subcloning” category belongs to “customized cloning”. The term “PCR cloning” is no longer used. From the perspective of GenScript’s pricing structure, only the subcloning performed on templates that were previously synthesized by GenScript is considered as “subcloning”. All cloning work carried out on customer provided template is considered “customized cloning”.
Articles in this section
- Why can't I cut this plasmid with XbaI and I can successfully cut other constructs with XbaI?
- Why customers get incorrect colonies when the CloneEZ reaction is transformed?
- Why customers are not able to get sufficient colonies when the CloneEZ reaction is transformed?
- Why do you add some extra bases outside of cloning sites? Do these bases interfere with any downstream applications?
- What's the method Genscript uses for subcloning?
- What is your definition of “subcloning” , “PCR cloning” and “customized cloning”?
- What is your cloning/subcloning method in generating constructs for customer?
- What is the main difference between cloneEZ and Invitrogen Gateway cloning?
- I need a common expression vector. Do you have any in stock?
- What E.coli competent do you usually use for cloning?