The concentration of PCR product is higher than 10ng/ul, and the plasmid concentration is higher than 50ng/ul. The concentration determined by the instrument is not necessarily accurate. We will confirm the sample size by comparing the 2ul run test with Marker comparison. We will sample the amount of 2ul absorbed by the sample to run the gel test and compare it to Marker to determine the additive volume.
Articles in this section
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
- Why is the sequence of primers in the sequencing results different from the expected sequence?
- Why is my gene sequence different from the standard sequence?
- Why I got a sequencing result that was not I want?
- Why does the sequencing primer need to be site-specific on the DNA template?