Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
Why there is a difference between my sequencing data and the one from the references?
Why the short PCR product is not suitable for direct sequencing?
Why target gene products are seen in control sample with no template?
Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
Why is the sequence of primers in the sequencing results different from the expected sequence?
Why I got a sequencing result that was not I want?
Why does the sequencing primer need to be site-specific on the DNA template?
Why does my sequencing fail?

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What are the reasons for the failure of sequencing?

August 13, 2019 12:30

(1) sequence failure caused by poor template quality.
(2) the primer and the sample can not be extended in combination.
(3) primers are not suitable for sequencing. Sequencing primers are more demanding than PCR primers. Random primers and degenerate primers can not be used for sequencing. Too long PCR primers are not suitable for sequencing.
(4) cause of primer mass.