When our technicians check the sequencing reports, usually we assume each peak is single and unique, so we might send you a report with a relatively stronger signal on the heterozygous site. If your samples do have a heterozygous site, please note it on the order submission. Our technicians will pay attention to this kind of sample. If the peak is indeed single on the putative heterozygous site, which indicates either an extremely low percentage of heterozygous DNA in your samples that is not detectable or it is a homozygous genotype.
Articles in this section
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
- Why is the sequence of primers in the sequencing results different from the expected sequence?
- Why is my gene sequence different from the standard sequence?
- Why I got a sequencing result that was not I want?
- Why does the sequencing primer need to be site-specific on the DNA template?