Every one of my purification products has been electrophoresis detection, and the strip running out can not be clearer. Why the sequencing test will not have a stripe?

For PCR samples, the concentration of agarose used in the company is 1% and the concentration of agarose at the time of recovery is 2%. Inconsistencies between your test results and your company's detection may be caused by: 1) The dye used in the sequencing test is bromphenylene blue GelRed, which has different buffer sensitivity than the one used by the customers. 2) In our company, the volume of the sample in the electrophoresis detection is 2ul. If your sample volume is greater than 2ul, the test results will be different from our test results. 3) Degradation of PCR products during transport due higher temperatures during transport. 4) Test is done without the same amount of detection marker. Please test with the same amount of detection marker to accurately know whether the concentration of the sample meets the requirements. Note: instrument measuring concentration might not be accurate. After receiving the samples, we will take 2ul samples to do gel electrophoresis detection, and compare the result with a marker to identify the concentration and amount of sample. Result should show a single clear and bright band.