For PCR samples, the concentration of agarose used in the company is 1% and the concentration of agarose at the time of recovery is 2%. Inconsistencies between your test results and your company's detection may be caused by: 1) The dye used in the sequencing test is bromphenylene blue GelRed, which has different buffer sensitivity than the one used by the customers. 2) In our company, the volume of the sample in the electrophoresis detection is 2ul. If your sample volume is greater than 2ul, the test results will be different from our test results. 3) Degradation of PCR products during transport due higher temperatures during transport. 4) Test is done without the same amount of detection marker. Please test with the same amount of detection marker to accurately know whether the concentration of the sample meets the requirements. Note: instrument measuring concentration might not be accurate. After receiving the samples, we will take 2ul samples to do gel electrophoresis detection, and compare the result with a marker to identify the concentration and amount of sample. Result should show a single clear and bright band.
Articles in this section
- Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
- Why there is a difference between my sequencing data and the one from the references?
- Why the short PCR product is not suitable for direct sequencing?
- Why target gene products are seen in control sample with no template?
- Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
- Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
- Why is the sequence of primers in the sequencing results different from the expected sequence?
- Why is my gene sequence different from the standard sequence?
- Why I got a sequencing result that was not I want?
- Why does the sequencing primer need to be site-specific on the DNA template?