For PCR samples, the concentration of agarose used in the company was 1%, and the concentration of agarose at the time of recovery was 2%. Inconsistencies between your test results and your company's detection may be caused by:
1) The dye used in the sequencing test is bromphenylene blue gelred, which has different buffer sensitivity as the yours.
2) In our company, the volume of the sample in the electrophoresis detection is 2ul. If your sample volume is greater than 2ul, the test results will be different from our test results.
3) Degradation of PCR products during transport due to inappropriate transport temperature.
4) Test with the same amount of detection marker to accurately know whether the concentration of the sample to meet the requirements.
Note: instrument measuring concentration might not be accurate. After receiving the samples, we will take 2ul samples to do gel electrophoresis detections and compare the result with the marker to identify the concentration and amount of the samples.