Will the primers degrade at room temperature during transportation? Can we use dry ice for transporting to maintain stability?
Why there is a difference between my sequencing data and the one from the references?
Why the short PCR product is not suitable for direct sequencing?
Why target gene products are seen in control sample with no template?
Why my sequence results are reverse complement to the template DNA, while I demand a 5’ to 3’ orientation?
Why is the TM value for primers of Genscript different from the TM value I obtained from other software?
Why is the sequence of primers in the sequencing results different from the expected sequence?
Why I got a sequencing result that was not I want?
Why does the sequencing primer need to be site-specific on the DNA template?
Why does my sequencing fail?

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Although the sample has been verified before, why the sequencing result would show an empty plasmid with inserted fragments?

October 20, 2023 06:57
Updated

Sequencing is the best proof of the sample. This might be due to:
1) The loss of insert fragments may occur during culturing, which can not be verified other than sequencing.
2) The sequenced clones are false positive clones.