Protein A/G chromatography purifies antibodies based on its general affinity for the constant region of IgG, while other affinity purification methods purify antigen-specific antibodies based on their affinity for the complementarity determining regions (CDRs). Usually for ELISA, WB, protein A/G purification is sufficient. For some other applications requiring high antibody specificity and purity like IHC, IP, etc, affinity purification is recommended. Sometimes even a combination of both purification methods might be necessary to yield extra pure antibody.
Articles in this section
- How do you validate the coverage of the HCP antibody? What is the sensitivity of HCP ELISA kit?
- Can you perform HCP antigen preparation? How do you validate?
- Do you have relevant experiences in ELISA kit development?
- Does GenScript have your own animal facility?
- How many positive hits could we guarantee in our beacon single b cell platform?
- Is GenScript able to keep the remaining positive B cells to backup?
- What are the benefits of express immunization compared to conventional immunization?
- What are the benefits of GenScript's MonoRab B cell cloning platform?
- How to decide what kind of immunogen format to use for antibody development?
- What kind of immunogens can we accept in upgraded TurboMab?