How do I re-constitute the lyophilized antibodies or antisera and the pre-immune serum?

Use deionized or distilled water for reconstituting the lyophilized products and mix gently by inverting 5-6 times at room temperature. Any insoluble matter present after reconstitution may be removed by centrifugation at 3000 rpm or higher for 10-15 min and the supernatant needs to be transferred to a fresh tube. This will not affect the performance of the antibody. For lyophilized antisera or preimmune sera, reconstitute to the original volume as mentioned on the data sheet or report sheet. For lyophilized purified antibodies, you may reconstitute to a final antibody concentration according to your applications or the recommended concentration on the data sheet.