According your situation, it seemed it was the high concentration of PBS caused the precipitation. We recommend to add water directly. The general rule of diluting stock solution is to add water (instead of PBS buffer) first, making sure the stock solution do not precipitate in water, then add 10X PBS buffer. The charge in PBS buffer causes precipitation sometimes.
Articles in this section
- Why it is difficult to synthesize peptide with FITC without the Ahx linker?
- I also noticed that one of the peptides was pinkish and others yellow.Could you please let me know the reasons?
- What’s the condition you suggest to store the peptide powder and the solution? Also, how long can they be store at room temperature?
- How to read my peptide QC report? Especially the HPLC and MS report?
- I would like to know what quality control measure you employ i.e., how do I determine that the product you sent me is what I wanted?
- How many continuous "R" can we synthesize in a peptide?
- Do you provide peptoid service?
- Can you cyclize my peptide by disulphide bond (cysteine bridge) while my peptide sequence contains 3 cysteines?
- What is your recommended peptide purity for in vivo application?
- For the peptide synthesis, can you send me the synthesis protocol that was used including the procedure that was used for making the disulfide bonds?