Boc is also N α-position protecting group for SPPS. Boc amino acid is attached to an insoluble support resin via a HF cleavable linker. Deprotection of Boc, is accomplished by treatment of the amino acid with TFA. The second Boc amino acid is coupled utilizing a pre-activated species or in situ activation. After the desired peptide is synthesized, the resin bound peptide is deprotected and detached from the solid support via HF cleavage. However, both N α-position protecting group and side chain protecting groups are acidic removable, and rounds of strong acid treatment would degrade the peptide, so this method is not very commonly used.
Articles in this section
- Why it is difficult to synthesize peptide with FITC without the Ahx linker?
- I also noticed that one of the peptides was pinkish and others yellow.Could you please let me know the reasons?
- What’s the condition you suggest to store the peptide powder and the solution? Also, how long can they be store at room temperature?
- How to read my peptide QC report? Especially the HPLC and MS report?
- I would like to know what quality control measure you employ i.e., how do I determine that the product you sent me is what I wanted?
- How many continuous "R" can we synthesize in a peptide?
- Do you provide peptoid service?
- Can you cyclize my peptide by disulphide bond (cysteine bridge) while my peptide sequence contains 3 cysteines?
- What is your recommended peptide purity for in vivo application?
- For the peptide synthesis, can you send me the synthesis protocol that was used including the procedure that was used for making the disulfide bonds?