Boc is also N α-position protecting group for SPPS. Boc amino acid is attached to an insoluble support resin via a HF cleavable linker. Deprotection of Boc, is accomplished by treatment of the amino acid with TFA. The second Boc amino acid is coupled utilizing a pre-activated species or in situ activation. After the desired peptide is synthesized, the resin bound peptide is deprotected and detached from the solid support via HF cleavage. However, both N α-position protecting group and side chain protecting groups are acidic removable, and rounds of strong acid treatment would degrade the peptide, so this method is not very commonly used.
Articles in this section
- What is the reason that amino acids are lost during the sequence synthesis? And how does GenScript solve it?
- For the peptide synthesis, can you send me the synthesis protocol that was used including the procedure that was used for making the disulfide bonds?
- Can you provide peptide on resin? What kind of resin you offer?
- What is the ratio of peptide to KLH or BSA when you do conjugation?
- What is the Boc strategy in SPPS?
- What is the Fmoc strategy in SPPS?
- In what direction are the peptides synthesized?
- What if there is a problem with the synthesis of my peptide?
- What methods do you use to synthesize peptides?
- What is the typical turnaround time for peptide synthesis at GenScript?