It is important to understand the difference between net peptide content and total peptide content. The dry peptide powder shipped to you usually contains not only peptide, but also some other substances such as water, absorbed solvents, counter ions and salts. The total peptide content(gross weight) refers to the weight of this mixture. Net peptide weight indicates the actual weight of the peptide component of your sample. Net peptide content is usually 50-80% of the total peptide weight (also called gross peptide weight) and is usually determined by amino acid analysis or UV spectrophotometry. Net peptide content should not be confused with purity. Purity defines the percentage of the target peptide sequence in the peptide component of your sample.
Articles in this section
- Why it is difficult to synthesize peptide with FITC without the Ahx linker?
- I also noticed that one of the peptides was pinkish and others yellow.Could you please let me know the reasons?
- What’s the condition you suggest to store the peptide powder and the solution? Also, how long can they be store at room temperature?
- How to read my peptide QC report? Especially the HPLC and MS report?
- I would like to know what quality control measure you employ i.e., how do I determine that the product you sent me is what I wanted?
- How many continuous "R" can we synthesize in a peptide?
- Do you provide peptoid service?
- Can you cyclize my peptide by disulphide bond (cysteine bridge) while my peptide sequence contains 3 cysteines?
- What is your recommended peptide purity for in vivo application?
- For the peptide synthesis, can you send me the synthesis protocol that was used including the procedure that was used for making the disulfide bonds?