It is important to understand the difference between net peptide content and total peptide content. The dry peptide powder shipped to you usually contains not only peptides, but also some other substances such as water, absorbed solvents, counterions, and salts. The total peptide content(gross weight) refers to the weight of this mixture. Net peptide weight indicates the actual weight of the peptide component of your sample. Net peptide content is usually 50-80% of the total peptide weight (also called gross peptide weight) and is usually determined by amino acid analysis or Nitrogen analysis. Net peptide content should not be confused with purity. Purity defines the percentage of the target peptide sequence in the peptide component of your sample.
Articles in this section
- Here is the sequence of the peptide 'QRPRLSHKGPMP', can you make it?
- What is the difference between Net Peptide Weight and Gross Peptide Weight?
- I know that you have technology to ligate small peptides into a long one. Can I send you my peptide fragments and have you ligate them together?
- Do you provide the Material Safety Data Sheet for us?
- What will be the maximum number of consecutive Pro peptide you can make?
- Do you provide reduced peptide?
- How do you calculate theoretical net peptide content?
- What is net peptide content?
- What is the maximum peptide length you can produce?
- Do I have to expect batch-to-batch variability?