Complementary primers can cause PCR artifacts, especially in control lanes (without template). The primers anneal to each other and extend, sometimes producing large amount of products. The extra bands that appear in the control lane usually will disappear in the presence of competing template. Depending on the degree of complementarity, raising the annealing temperature may help if there are extra bands in other lanes.
Articles in this section
- Why do I see extra bands in my PCR product?
- Which vector do you use when synthesizing the gene?
- Which software can I use to open the sequence files of the synthesized gene?
- What QC data do I get for gene synthesis service?
- What options does GenScript offer for gene deliverables?
- What kind of bacterial strain is used in gene synthesis service and how should it be handled?
- What is the storage condition of genes/ constructs you made?
- What is the format you deliver the gene?
- How much does it cost if I want to synthesize a gene and clone it to a vector?
- What is the biggest gene you can synthesize?